Influence of formaldehyde and urea on the enzymatic hydrolysis of cellulose with the application of industrial enzymes

Wood industry generates many waste in the form of postconsumer materials. Utilization costs are high. Curing of receivers, who know the manner of such a material processing would be the most profitable way of this problem solution. It would not only allow the costs reduction, but could bring possible profits. Poland is the country which is rich in biomass originated from postconsumer wood materials.
The opportunity of processing of wood biomass from postconsumer boards to biofuels gives the chance to utilize material which wasn’t used in other industry branches. At the same time it doesn’t pollute the natural environment with wastes storage and resins decomposition, as well as releasing of harmful substances during wastes burning. Particle board glued with urea-formaldehyde resin is the most popular wood material used in Poland.
In this paper contents of following components of pine wood were determined: cellulose with Kürschner-Hoffer method, holocellulose with sodium chlorite method and monosaccharides with the application of 72% H2SO4. Wood chips were extracted with the mixture of chloroform and ethanol (93:7w) before the analysis.
The influence was studied of different urea and formaldehyde addition to standard cellulose (Whatman No.1 filter paper strip) on the enzymatic hydrolysis efficiency to decide if any of them inhibits the process. Additionally hydrolyses were performed on the native chips and chips gained from model particleboards. The enzymatic hydrolyses were carried out with the application of industrial enzymes Novozymes CTEC2. Saccharides content in obtained hydrolyzates were determined with chromatographic analysis (HPLC).

Keywords: pine, particle board, biomass, enzymatic hydrolysis

Authors

Lewandowska A.
Department of Wood Science and Wood Protection, Faculty of Wood Technology, Warsaw University of Life Sciences – SGGW

Marchwicka M.
Department of Wood Science and Wood Protection, Faculty of Wood Technology, Warsaw University of Life Sciences – SGGW

Auriga R.
Faculty of Wood Technology, Warsaw University of Life Sciences – SGGW

Radomski A.
Department of Wood Science and Wood Protection, Faculty of Wood Technology, Warsaw University of Life Sciences – SGGW

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